In Vitro Effect of the Fungicide Fitoklin on Spore Germination and Mycelial Growth of Trichoderma asperellum

Abstract

The main objective was determining the in vitro tolerance between Trichoderma asperellum and the fungicide Fitoklin. Therefore, the effect of the fungicide on germination and the growth of T. asperellum was evaluated. The evaluated fungicide concentrations were 262.5, 350.0 (field dose), and 437.5 mg/L. The results showed that none of the three concentrations inhibited the germination of T. asperellum spores since the growth and pigmentation characteristic of the fungus occur. On the other hand, as the concentrations of the fungicide increased, the growth of the fungus was inhibited; however, it was able to tolerate the fungicide, which according to the existing literature, T. asperellum may express genes of ABC transporters and oxidoreductases, which play an essential role in the degradation of pesticides. Therefore, it is concluded that, in vitro, T. asperellum can be tolerant with the fungicide Fitoklin, which can be considered if it is to be applied jointly in integrated pest management (IPM), which, in turn, represents a sustainable way with the environment. which can be considered when applying them together IPM, which, in turn, represents a sustainable way with the environment.